Graduate Seminar Series: Cell and Tissue Stream – Aaron Rosenstein

Graduate Seminar Series: Cell and Tissue Stream
Graduate Seminar Series for the Institute of Biomedical Engineering (BME). This day is for cell and tissue stream presenters.
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Presentation Title: Recording cellular histories in DNA with prime editing
Abstract: Recording cellular stimuli into DNA has been a common aspiration of synthetic biologists for the purposes as both a research tool and eventual clinical diagnostic application. Whether as a means of understanding interactions on the single-cell level, or reconstructing histories of cellular events, a cellular DNA recording device has widespread utility. Previous attempts at building a robust recorder, however, have fallen short on this goal. Here I present steps taken towards developing a recording device which can incorporate sequential analog signals of events into genomic DNA. This device will leverage prime editing as a means of integrating signals encoded on prime editing guide RNAs (pegRNAs) into a specific region of the genome termed recording loci, while pegRNA expression will be induced by the magnitude of signals respectively. I envisage a mechanism whereby repeated prime editing can construct an organized cellular record of event histories. Development of this device requires empirical identification of the sequence-determinants of effective prime editing, as well as constructs for translation of diverse cellular signals into pegRNA messages of commensurate magnitude. Preliminary results indicate a baseline for recording synthetic signals in this manner. Ultimately this project will yield a robust recording device which can meet investigator’s needs, and can hopefully be adopted as both a research tool and clinical diagnostic device.
Supervisor Name: Dr. Michael Garton
Year of Study: 2
Program of Study: PhD
Zoom link: https://us02web.zoom.us/j/89610372821?pwd=azd4SCtYVWtreVovaGNPV1c2NGY2Zz09
Meeting ID: 896 1037 2821
Password: 483329
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