Canceled: Graduate Seminar Series: Clinical Stream – Yufeng Wang

Graduate Seminar Series: Clinical Stream
Graduate Seminar Series for the Institute of Biomedical Engineering (BME). This day is for clinical stream presenters.
If you would like to invite your Principal Investigator, please add their email via the ‘Add Guest’ button and they will also be notified of your presentation.
Presentation Title: Design of an Islet-on-a-Chip Device to Dynamically Measure Insulin Secretion from Individual Islets
Abstract: Glucose-stimulated insulin secretion (GSIS) from pancreatic islets shows a biphasic pattern. Investigation of temporal dynamics and patterns of GSIS could give insights into mechanisms underlying β-cell function and dysfunction. Here, we present a novel microfluidic device to detect insulin secretion from individual islets on-chip through miniaturizing a fluorescence anisotropy immunoassay (FAIA). Insulin is co-secreted with c-peptide within ~5-10 min of glucose stimulation. Here we designed a c-peptide sensor with a fluorophore tagged to a synthetic c-peptide fragment and optimized assay parameters to maximize the sensitivity of the FAIA. Subsequently, a continuous-flow method was applied to monitor reaction kinetics to determine the equilibrium time of the assay. Using the maximum time required to reach equilibrium, we designed an islet-on-a-chip that captures the islet effluent and sufficiently mixes it with the reagents within the ~100 s residence time. This assay is downstream of the islet and leaves an ideal optical window to simultaneously image islet responses (e.g., Ca2+ activity). Ultimately, this device was used to measure c-peptide secretion from 4 individual mouse pancreatic islets simultaneously. The secretion detected on-chip shows the characteristic biphasic pattern and was further validated with pharmacological activators (ex. tolbutamide) for insulin secretion. In the future, the islet-on-a-chip could be coupled with other sensors, such as Ca2+ and oxygen consumption rate sensors to further probe β-cell metabolism.
Supervisor Name: Dr. Jonthan Rocheleau
Year of Study: 4
Program of Study: PhD
Zoom link: https://us02web.zoom.us/j/89610372821?pwd=azd4SCtYVWtreVovaGNPV1c2NGY2Zz09
Meeting ID: 896 1037 2821
Password: 483329
Cancellation Reason:
Powered by Calendly.com